Pharma IQ Glossary: Complementary DNA (cDNA)
Complementary DNA (cDNA) is DNA synthesized from a mature mRNA template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase. cDNA is often used to clone eukaryotic genes in prokaryotes. When scientists want to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell. cDNA is also produced by retroviruses (such as HIV-1, HIV-2, Simian Immunodeficiency Virus, etc.) which is integrated into its host to create a provirus.
Why Not Sequence the Whole Genome?
September 13 by Andrea CharlesProfessor René Bernards, Head of the Division/Group Leader, Molecular Carcinogenesis at The Netherlands Cancer Institute, joins Andrea Charles from Pharma IQ, to discuss current trends in next-ge...
How to Overcome Challenges with siRNA Targeting
December 13 by Pharma IQProfessor Ann Logan, Lead Professor, University of Birmingham, joins Helen Winsor Pharma IQ, to discuss next-generation DNA sequencing. Logan reports on her laboratory’s primary research into the...
Using Next-Generation DNA Sequencing Methods
August 08 by Pharma IQAlthough more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA ...
