Pharma IQ Glossary: DNA Sequencing
DNA Sequencing refers to sequencing methods for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA. Two common methods available are The Maxam Gilbert technique, which uses chemicals to cleave DNA into fragments at specific bases; or, most commonly, the Sanger technique (also called the di-deoxy or chain-terminating method) which uses DNA polymerase to make new DNA chains, in the presence of di-deoxynucleotides (chain terminators) to stop the chain randomly as it grows. In both cases, the DNA fragments are separated according to length by polyacrylamide gel electrophoresis, enabling the sequence to be read directly from the gel.
An Interview with Gerd Maass, VP for R&D at Roche Pharmaceuticals: Chapter 1 - DNA Sequencing
April 13 by Pharmaceuticals & Biotechnology EditorGerd Maass, Vice President for R&D at Roche Pharmaceuticals, shares his views on advances in companion diagnostics, DNA sequencing and biobanking. This segment on DNA sequencing addresses claims t...
Accelerating Biological and Biomedical Research
November 30 by Pharma IQThe outlook for genome research looks bright. A technological evolution in the past few years has reduced the cost of DNA sequencing, accelerating the possibilities for future rese...
Using Next-Generation DNA Sequencing Methods
August 08 by Pharma IQAlthough more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA ...
